Part:BBa_I7100:Experience
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Applications of BBa_I7100
User Reviews
UNIQ725e4c2f7dd390de-partinfo-00000000-QINU
Antiquity |
This review comes from the old result system and indicates that this part did not work in some test. |
Kahaynes |
This part is supposed to be in well 10K of iGEM 2006 DNA-2, but what is actually in this well is probably a completely different part. We cloned this part in E. coli strain JM109 and saw no GFP expression. The vector is supposed to be pSB3K3 (2750 bp) and the insert should be ~950 bp, but a restriction digest showed the vector to be > 3000 bp and the insert is > 1000 bp. This plasmid should convey Kan resistance only, but the clones are both Amp and Kan resistant. The digests used to confirm the plasmids at each step looked clean (two clear bands) so the content of well 10K of iGEM 2006 DNA-2 is probably a BioBrick part, just not the right vector or part. The vector is probably pSB*AK*. --Kahaynes 15:45, 23 October 2006 (EDT) |
••••• |
BBa_I7100 produced constitutive fluorescence in the absence of TetR. |
UNIQ725e4c2f7dd390de-partinfo-00000005-QINU
Characterization
Transcriptional control of GFP generator
[Note: BBa_I7100 is a composite part of BBa_R0040 and BBa_E0840.]